Description

Performance: - Fluorescence Sensitivity - FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) - PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE) - PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5) - APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC) - FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A) - PE-Cy5 and APC measurements performed using SPHERO™ Ultra Rainbow Calibration Particles (URCP38-2K) Fluorescence Resolution: - Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN) Fluorescence Linearity: - Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser Forward and Side Scatter Sensitivity: - Enables separation of fixed platelets from noise. Forward and Side Scatter Resolution: - Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes. Side Scatter Resolution: - Enables separation of 0.5-μm beads from noise. Data Acquisition Rate: - 40,000 events/sec with beads Fluidics: - Sample Flow Rates: Front button panel provides three modes: RUN, STANDBY, and PRIME - Continuously adjustable flow rate, plus three preset flow rates: LO: 12 μL/min; MED: 35 μL/min; HI: 60 μL/min - Standard Fluidic Reservoirs: One 8-L sheath container and one 10-L waste container provided. Data Management Software: BD FACSDiva™ v8.0 or later Dimensions: 29″ W x 30″ D x 30″ H inches (73.7 x 76.2 76.2 cm) Weight: ~159 kg (~350 lb) depending on configuration Temperature Operating Range: 19°C–26°C Humidity: 10% to 90% relative, non-condensing Heat Dissipation: ~2,700 BTU per hour depending on configuration Air Supply: None required Power: 100-230 V, 60 Hz. The unit (including computer system/printer) requires one dedicated circuit with a dedicated AC source not shared with any other equipment. Maximum power: 1,500 watts Noise: <70 dB Compliance with Safety Standards: UL 61010 (US)

Configuration

Specifications: Optics: - Excitation Optics - Excitation Optical Platform - The BD LSRFortessa X-20 optical layout allows for up to five lasers. Laser Power: - 355 nm: 15 mW - 405 nm: 50 mW - 488 nm: 50 mW - 561 nm: 50 mW - 640 nm: 40 mW Optical Efficiency: - Power loss at flow cell: <20% of specified laser power Flow Cell Design: - Rectangular quartz cuvette: Internal cross-section, 430 x 180 μm - External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light. - Fixed optical assembly with spatially separated laser beams. Emission Optics: - Optical Coupling - The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency. Forward Scatter Detection: - Photodiode detector with a 488/10 bandpass (BP) filter Side Scatter Detector: - Photomultiplier tube (PMT) with a 488/10 BP filter Emission Optical Design: - Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. - The BD LSRFortessa X-20 uses configurable polygon-shaped optical pathways that use signal reflection to maximize signal detection.